The western blotting method was proposed first by Towbin in 1979 as we mentioned above. Process of Western Blot Experiment Introduction If you want to learn more virtual labs and its benefits, check out my review of this topic. Watch the walkthrough video and get a full understanding of how to conduct the western blot simulation in PraxiLabs’ Virtual Science Labs. The bound and labeled protein can be visualized giving information about its molecular weight and relative quantity. nitrocellulose or PVDF membrane).įinally, a specific antibody is used to detect the protein of interest. Then, the proteins are electrophoretically transferred (blotted) to a more durable surface (e.g. Proteins in the sample are extracted then separated by gel electrophoresis (SDS-PAGE) according to their molecular weight. Western blot is a commonly used technique designed to study a specific protein in a sample containing many other proteins.
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